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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in lots of tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical job in advertising and marketing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two added splice variants were being reported but comprehensive nucleotide sequence info just isn't nonetheless offered.
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Blocking peptides are peptides that bind particularly to your goal antibody and block antibody binding. These peptide commonly includes the epitope identified by the antibody.
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This manual outlines even further feasible optimization of cellular and immunoassay parameters to guarantee the absolute best final results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs for the remarkably conserved cyclin spouse and children, whose members are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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